Determination of ethinylestradiol and norgestrel in water using solid-phase microextraction

  • Mereke Baurzhanovna Alimzhanova al-Farabi Kazakh National University, Almaty
  • Yerlan Adilbekov al-Farabi Kazakh National University
  • Yenglik Nurzhanova al-Farabi Kazakh National University
  • Svetlana Batyrbekova al-Farabi Kazakh National University
Keywords: ethinylestradiol, water sample, gas-chromatography, mass-spectrometry, solid-phase microextraction


Endocrine disrupting compounds (EDCs) are exogenous substances that alter some function of the endocrine system and have adverse health effects on intact organisms, their off spring or (sub)populations. EDCs mimic or block the action of natural hormones, and hence biological functions in living organisms, thus leading to impaired reproduction, growth and development. The article focuses on the development of analytical method based on solid-phase microextraction and gas chromatography – mass spectrometry for detection of steroid hormones ethinylestradiol and norgestrel in water samples. In this work, studies were carried out on model water samples spiked with a mixture of ethinylestradiol and norgestrel. The following parameters of headspace solid-phase microextraction were optimized as a result of experiments: extraction temperature 80°C, extraction time 20 min, NaCl additive – 1.5 g, fiber coating – 65 µm PDMS/DVB. 

Author Biographies

Mereke Baurzhanovna Alimzhanova, al-Farabi Kazakh National University, Almaty

Center of Physical Chemical Methods of Research and Analysis

Yerlan Adilbekov, al-Farabi Kazakh National University
Center of Physical Chemical Methods of Research and Analysis
Yenglik Nurzhanova, al-Farabi Kazakh National University
Center of Physical Chemical Methods of Research and Analysis
Svetlana Batyrbekova, al-Farabi Kazakh National University
Center of Physical Chemical Methods of Research and Analysis


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How to Cite
Alimzhanova, M., Adilbekov, Y., Nurzhanova, Y., & Batyrbekova, S. (2016). Determination of ethinylestradiol and norgestrel in water using solid-phase microextraction. Chemical Bulletin of Kazakh National University, 83(3-4), 52-62.