Ophthalmic drug delivery system based on the complex of gellan and ofloxacin

Keywords: gellan, ofloxacin, complexation, drug delivery

Abstract

Complex formation between a natural polysaccharide – gellan and an antimicrobial drug – ofloxacin was studied in aqueous solution. Conductimetric and potentiometric titration curves revealed that gellan and ofloxacin forms a water-soluble complex of composition 2:1 mol/mol stabilized by ionic and hydrogen bonds. The formation of the gellan-ofloxacin complex was confirmed by FTIR spectroscopy, dynamic light scattering, zeta-potential and thermogravimetric analysis. The average hydrodynamic size of the complex was found 307±5nm and its zeta-potential was negative and equal to -15 mV. Thin films of the gellan-ofloxacin complex, gelled in 0.3 wt.% of CaCl2, were used to study the release kinetics of ofloxacin in distilled water and phosphate buffer. The drug release kinetics evaluated by UV-Vis spectroscopy at λmax = 289 nm and calculated by the Ritger-Peppas model correspond to non-Fickian diffusion in distilled water and Case II transport (zero-order kinetics) in phosphate buffer. The cumulative release of ofloxacin from the gellan-ofloxacin films was equal to 96±2% and 36±2% in phosphate buffer and distilled water, respectively. It is expected that the gellan-ofloxacin complex is able to form in situ gel on the surface of the eye and to prolong the drug residence time in the tear fluid.

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Published
2022-06-20
How to Cite
Tatykhanova, G., Aseyev, V., Vamvakaki, M., Khutoryanskiy, V., & Kudaibergenov, S. (2022). Ophthalmic drug delivery system based on the complex of gellan and ofloxacin. Chemical Bulletin of Kazakh National University, 105(2), 4-12. https://doi.org/https://doi.org/10.15328/cb1239