Confirmation of 17b-methyl-5b-androst-1-ene-3a,17a-diol metabolite of methandienone in urine by triple quadrupole mass-selective detector gas chromatography (GC/MS/MS) method
Keywords:
anabolic substances, epimetendiol, urine, gas chromatography, doping
Abstract
This article shows the sample preparation used in the analysis, consisting of cleaning of the urine from organic and inorganic chemicals, hydrolysis with β-glucuronidase, extraction with n-pentane and derivatization. Following equipment was used during the performance of this work: a gas chromatograph Agilent Technologies 7890A GC System with 7693 Autosampler and mass selective detector Triple Quad 7000 GC/MS. GC/MS/MS chromatograms of epimetendiol obtained by this method were found to be appropriate. It is shown that the retention time and piek of epimetendiol is 11.29 minute. This method allows confirming epimetendiol's urinary concentration of 1-2 ng/mL.References
1 Schаnzer W. Metabolism of anabolic androgenic steroids // Clinical Chemistry. – 1996. – V.1020, № 3. – P. 1012-1013.
2 Schаnzer W., Donike M. Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolites // Analytica Chimica acta. – 1993. – V. 275, № 3. – P. 23-48.
3 Schanzer W., Delahout P., Geyer H., Machnik M., Horning S. Long-term detection and identification of metandienone and stanozolol abuse in athletes by gas chromatography-high-resolution mass-spectrometry // Journal of chromatography B. – 1996. – V. 687, Is. 4. P.93-108.
4 Gotzmann A., Geyer H., Schanzer W. HPLC Clean-up for urine samples with disturbing background for confirmation of 17β-methyl-5β-androst-1-ene-3α,17α-diol (Epimetendiol) // Recent advances in doping analysis. – 1996. – Vol. 4. – P.239-244.
5 WADA Technical Document – TD2013MRPL Minimum required performance levels for detection and identification of non-threshold substance.
2 Schаnzer W., Donike M. Metabolism of anabolic steroids in man: synthesis and use of reference substances for identification of anabolic steroid metabolites // Analytica Chimica acta. – 1993. – V. 275, № 3. – P. 23-48.
3 Schanzer W., Delahout P., Geyer H., Machnik M., Horning S. Long-term detection and identification of metandienone and stanozolol abuse in athletes by gas chromatography-high-resolution mass-spectrometry // Journal of chromatography B. – 1996. – V. 687, Is. 4. P.93-108.
4 Gotzmann A., Geyer H., Schanzer W. HPLC Clean-up for urine samples with disturbing background for confirmation of 17β-methyl-5β-androst-1-ene-3α,17α-diol (Epimetendiol) // Recent advances in doping analysis. – 1996. – Vol. 4. – P.239-244.
5 WADA Technical Document – TD2013MRPL Minimum required performance levels for detection and identification of non-threshold substance.
Published
2013-05-14
How to Cite
Shorabekova, U., Balgimbekova, K., Shaldybayeva, A., & Talbayev, T. (2013). Confirmation of 17b-methyl-5b-androst-1-ene-3a,17a-diol metabolite of methandienone in urine by triple quadrupole mass-selective detector gas chromatography (GC/MS/MS) method. Chemical Bulletin of Kazakh National University, 69(1), 44-50. https://doi.org/https://doi.org/10.15328/chemb_2013_144-50
Section
Analytical Chemistry
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License (CC BY-NC-ND 4.0) that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
